The HLA B5701 Strip test allows the determination of the HLA B5701 alleles.
Since the introduction of combined antiretroviral therapy, HIV infection has gone from being a serious and fatal disease to one which is chronic but controllable. Antiretroviral therapy usually entails combining 2 nucleosides which are analogous to reverse transcriptase inhibitors and either an HIV protease inhibitor or a non-nucleoside reverse transcriptase inhibitor. These drugs are very effective but they are associated with a series of undesired effects, such as severe hypersensitivity reactions, especially in the case of therapies using abacavir or nevirapine.
Abacavir is used in the treatment of HIV-1 infection since 1999. The WHO recommends abacavir as a second-line treatment, owing to the risk of hypersensitivity associated with its use, which affects 5-8 % of Caucasian patients.
Several studies have demonstrated the existence of a strong predictive correlation, in Caucasian populations and groups of Hispanic ethnicity, between hypersensitivity to abacavir and the presence of the HLA-B5701 allele. This correlation is strong enough to enable us to predict the risk of hypersensitivity to abacavir and classify individuals as low (<1%) or high (>70 %) risk, based on the absence or presence of the HLA-B5701 allele.
Several polymorphisms has been identified. These ones explain nearly 15 % of the variability in HIV-1 viral load in individuals during the asymptomatic phase of the disease were identified. One of them, located in the HCP5 gene (HLA complex P5), shows a perfect linkage disequilibrium with HLA-B5701 (r2 = 1)..
The useful role of this SNP in predicting hypersensitivity to abacavir has been demonstrated in European populations (100 % detection sensitivity for HLA-B5701, specificity of 99.4 %, positive predictive value of 94.2 % and negative predictive value of 100 %).
|HLA B5701 Strip||Hybridization on Strip||ADN|
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